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KMID : 0043319980210020212
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Archives of Pharmacal Research
1998 Volume.21 No. 2 p.212 ~ p.216
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Coupled Column Chromatography in Chiral Separation of Salmeterol
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Kim Kyeong-Ho
Yun Hyeong-Won
Kim Hyun-Ju
Park Hyun-Ji
Choi Pok-Wha
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Abstract
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A coupled achiral-chiral high-performance liquid chromatographic system has been developed for the determination of the enantiomers of salmeterol, S-(+)-salmeterol and R-(-)-salmeterol in urine. THe salmeterol was separated from the interfering components in urine and quantified on the silica column, and the enantiomeric composition was determined on a Sumichiral OA-4700 chiral stationary phase. The two columns were connected by a switching valve equipped with a silica precolumn. The two columns wer connected by a switching valve equipped with a silica precolumn. The precolumn was used to concentrate the salmeterol in the eluent from the achiral column before backflushing onto the chiral phase. The coupled system was validated.
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KEYWORD
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Chiral separation, HPLC, Coupled column chromatography, Salmeterol, Enantiomer, Column switching
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